GST瓊脂糖凝膠4FF/谷胱甘肽瓊脂糖凝膠4FF/Glutathione Sepharose 4FF

英文名稱：Glutathione Sepharose 4FF
其他名稱：谷胱甘肽瓊脂糖凝膠4FF

級別：BR
Ligand：glutathione and 10-carbon linker arm
Ligand concentration：120-320umol glutathione/ml medium
Binding capacity：～10mg recombinant glutathione S-transferase/ml medium,GST Mr26000
Dynamic binding capacity：～11mg GST-tagged protein/ml medium Mr43000（GSTrap FF 1ml at 1 ml/min=156cm/h）
Mean particle size：90um
Bead structure：highly cross-linked 4% agarose
Maximum flow rate：450cm/h（15ml/min）,using xk16/20 column with 5cm bed height,run at room temperature with aqueous buffer
Recommended flow rate：Sample loading:＜100cm/h（＜3ml/min using XK16/20 column）；Washing and elution:100～300ml/min using XK16/20 column）
PH stability：3-12
Chemical stability：All commonly used aqueous buffers,e.g. 1M acetate pH 4.0 and 6M guanidine hydrochloride for 1 hour at room temperature
性狀：含20%乙醇，底部為乳白色膠體
用途：生化研究。專門用于純谷胱甘肽S-轉移酶（GST）融合蛋白、其他的谷胱甘肽轉移酶以及與谷胱甘肽有親和作用的親和色譜填料，一步分離就可得到很純的目標蛋白，純化條件溫和，可保證蛋白的活性
保存：2～8℃